![]() ![]() įixation is a process which is necessary to prevent tissue from diffusion of soluble component there by prevent autolysis and putrefaction. reusable tissue cassette if not cleaned properly can carry the older tissue specimen fragment. these starch granule can be misinterpreted with pyknotic nucleus. Starch powder present in the gloves lead to starch artifact. The presence of foam gel in histological section can cause distorted space filled by blood surrounded by slightly basophilic gelatin wall sometime foam gel is used to control bleeding. Presence of suture material in microscopic tissue is also common artifact which causes damage to the microtome knives and leads to tear in tissue section. This can be avoided by using knives and electrical point. Heat from the laser used for biopsy may lead to detachment of epithelial cells, detachment of epithelium from the basement membrane also induce carbonization, nuclear elongation and vacuolar degeneration. Electrocautry in parotid surgery causes oncocytic changes in acinar cell If electrocautry are used for coagulation can drive surface epithelium into the connective tissue thus producing a pseudocysts and loss of cytoplasmic and nuclear feature. Some of the commonly used surgical instrument for the biopsy can penetrate the specimen and it can cause voids or tears and compression of surface epithelium into the connective tissue. It can be avoided by using block injection technique or injecting well away from the site. ![]() Large amount of anesthetics solution injected into site can lead to hemorrhage, vacuolation of epithelium and it may separate the connective tissue from epithelium. These colored solution are used carefully and this should be mentioned clearly because it may interfere tissue processing and staining procedure. Sometime tincture iodine is used as decontaminant for biopsy site. Some commonly used reagents are Indian blue,alcian blue or alcian green ,silver nitrate. ![]() These types of artifact are produced before the tissue is being fixed.Some of the coloring agent is used for marking the biopsy site and for appropriate orientation of the specimen margins. Artifact can occurs at any stage, which can be classified as the following Presence of artifact can lead to inaccurate diagnosis.Hence,it is important to know and understand the commonly occurring artifact, so that we can avoid misdiagnosis. when it is present it may confuse with normal tissue or any pathological changes. Some artifact are easily distinguished and some are not. Available from: Īn artifact is defined as any structure or feature in the histological section, which is not normally present in the normal tissue, and it may come from outside sources. Histological section pathological embedding microtomyĭownload this article as: Copy the following to cite this article: It is therefore important to identify the common occurring artifacts during interpretation of oral biopsies. These artifacts may occur during surgical removal, fixation, processing, embedding, microtomy and staining procedures. Defects are common in this lengthy procedure and they are referred as Artifact.Accuracy of the histopathological diagnosis depends on eliminating or minimizing the artifact in histological section. processing of tissue specimen is a length procedure. Microscopic examination of histological section of the tissue does not always related to normal histology or pathology. Balachanderĭepartment of Oral Pathology, Sree Balaji Dental College and Hospital, Bharath University, Pallikaranai, Chennai-600100. ![]()
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